Animal Cell Technology: Products of Today, Prospects for by R. E. Spier, J. B. Griffiths, W. Berthold

By R. E. Spier, J. B. Griffiths, W. Berthold

Most vital for practicable and wealthy animal mobilephone expertise is the fulfillment of profitable items hence developing gain for sufferers and credibility for the industrial allure of this fairly younger expertise. The papers offered during this quantity deal with the newest matters and glance to destiny advancements within the fields of animal mobilephone expertise. very important subject matters thought of in those shows comprise downstream processing and regulatory defense points. The twelfth ESCAT assembly lawsuits proceed to supply an entire assessment of this significant subject and should be a useful reference resource for these interested by the construction and use of animal cells.

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3. 16 Cells were inoculated into 1 ml of PEG-86-1 medium and incubated at 37°C in a CO2 incubator ( · ; total cell number, O; viable cell number, ■ ; anti-HBsAg h-IgG, D ; nonspecific h-IgM (b) Introduction of the Tac gene Gene technolohy has opened the way for manufacturing various useful proteins by mammalian cells as well as microorganisms , and it is expected that gene technology can also be used to improve growth characteristic s or production capacity (8). Human interleukin-2 (IL-2) was discovered as a growth factor for T-cells, but it also affects the growth and differentiation of B-cells.

The productivity was also improved by introducing an lnterleukin-2 receptor(Ta c antigen) gene. By introducing the human basic fibroblast growth factor gene, seed culturing steps have been simplified. An effective manufacturing system for human nerve growth factor has also been establishe d using a serum-free suspensio n culture of recombinan t CHO cells. Keywords: cell-line improvement, human hybridoma, heterohybridoma , CHO cells, human monoclonal antibody, human NGF, serum-free culture, large-scale culture, HBsAg, tetanus toxoid 1.

Acknowledgements. C. G. are very grateful to Dr J. Stephenson for use of facilities, and to Mr. A. Fooks for advice and assistance with plasmid preparation. 51 Table 1. Immortalised cell lines-CPE caused by test viruses [% cultures showing CPE (total)] Parainfluenza 2. Parainfluenza 3. 100(8) 76(21) 100(6) 100(4) 100(8) 100(7) 88(18) 78(14) 71(14) 75(8) 50(8) 35(20) 33(6) 80(5) 60(10) 71(7) 41(17) 30(13) 50(12) 45(11) 50(8) 57(21) 33(6) 60(5) 60(10) 57(7) 58(17) 60(13) 83(12) 36(11) 75(8) 60(20) 20(5) 60(5) 60(10) 71(7) 56(16) 60(13) 75(12) 10(10) I 42/17/11 42/17/12 42/17/17 I 42/17/22 100(8) 100(8) 100(2) 100(6) 38(8) 38(8) 100(1) 50(6) 38(8) 63(8) 100(1) 50(6) 0(9) 25(8) 100(1) 60(5) I dt12D2 dt12D3 dt12D4 dt12D10 dt12D12 dt12D13 dt12D14 dt12D17 100(7) 100(2) 100(9) 0(1) 100(1) 100(11) 100(3) 0(1) 17(7) 50(2) 88(9) ND 100(1) 60(10) 75(4) 0(1) 75(6) 100(2) 70(10) 0(1) ND 63(11) 100(3) 0(1) 42(7) 50(2) 80(10) 0(1) ND 72(11) 100(3) 0(1) I dt11/7 dt11/11 dt11/16 100(1) 150(2) 100(3) 100(1) 50(2) 67(3) 0(1) 50(2) 67(3) 0(1) 0(2) 67(3) I ori-11/7 ori-11/9 ori-11/11 ori-11/16 ori-11/17 ori-11/17a I ori-11/19 75(4) 100(19) 100(7) 100(3) 100(5) 100(2) 100(1) 50(4) 79(19) 85(7) ND 80(5) 100(2) 100(1) 75(4) 73(19) 85(7) ND 80(5) 100(2) 0(1) 75(4) 52(19) 85(7) 100(3) 80(5) 50(2) 0(1) I I PBK 100(15) 88(16) 88(16) 93(15) I Cell Line Coxsackie B3 42/17/1 42/17/3 42/17/3a 42/17/4 42/17/4a 42/17/4b 42/17/6 42/17/7 42/17/8 42/17/9 52 Influenza A Taiwan | I Table 2.

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